EBTT 2015

The meeting

Cell membrane electroporation — mechanisms & applications

Cell membrane electroporation, its mechanisms and applications in biology, medicine and biotechnology. The basis of the phenomena, current applications and future prospects were the main topics of this international meeting.

The postgraduate course was intended both for novices and experts in electroporation, including PhD students, researchers and users. Abstract submission was strongly encouraged and was necessary if credits (5 ECTS) were sought — each participant submitting an abstract was also required to give a short oral presentation of the work. This course was accredited also by the Medical Chamber of Slovenia, so active and passive participants received 20 CME credits.

Read all about EBTT in an article published by MediaResearch Ltd (2012). International Innovation is the leading global dissemination resource for the wider scientific, technology and research communities — more at www.researchmedia.eu.

Organised by

University of Ljubljana · Institute of Oncology, Ljubljana · COST Action TD 1104

Organising committee

Chair: Peter Kramar
Members: Matej Kranjc, Marija Marčan, Tadeja Forjanič, Saša Haberl, Duša Hodžić, Bor Kos

Alumni

Ambassadors of the EBTT

Paulo A. Garcia, Ph.D.

Massachusetts Institute of Technology (MIT), 77 Massachusetts Avenue (5-021), Cambridge, MA 02139 USA
web.mit.edu/lemi

Dr. Boštjan Markelc

Oxford Institute for Radiation Oncology, Department of Oncology, University of Oxford, Old Road Campus Research Building, Roosevelt Drive, Oxford OX3 7DQ, United Kingdom

Dr. Aude Silve

Karlsruher Institut für Technologie (KIT), Institut für Hochleistungsimpuls- und Mikrowellentechnik (IHM), Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany
www.kit.edu

Course content

Key lectures & topics

Electrical properties of cells & tissues

Resting & induced transmembrane voltage; dielectric properties of tissues; temperature & conductivity changes.

Physical chemistry of electroporation

Electrochemical membrane thermodynamics; molecular dynamics simulations.

Electroporation in vitro & in vivo

Protocols for electroporation of cells in vitro and in vivo.

Devices & electrodes

Devices & electrodes for in vitro / in vivo work; tissue models & their experimental validation.

Electrochemotherapy of tumours

Basic mechanisms, preclinical studies, clinical use, current developments & future perspectives.

Gene transfection

Gene transfection in vitro / in vivo; clinical use in human medicine.

Transdermal drug delivery

Electroporation for gene delivery in the skin; DNA vaccines.

Additional topics

Delivery of biomolecules & nanoparticles; ECT in clinical practice; bioimpedance; PEF food processing; multiphoton optogenetic imaging.

Additional invited talks

Pouyan BoukanyNon-endocytic delivery of biomolecules and nano-particles by electroporation
Julie GehlElectrochemotherapy in clinical practice; lessons from development and implementation — and future perspectives
Antoni IvorraElectrical bioimpedance and electroporation
Henry JägerPulsed electric field application in food processing — equipment design and matrix properties
Rodney P. O'ConnorMultiphoton imaging with optogenetics tools to assess bioelectric effects in vivo

Faculty

Directors & lecturers

Co-DirectorDamijan Miklavčič

Co-DirectorLluis M. Mir

Faculty members & lecturers

Damijan MiklavčičUniversity of Ljubljana, Slovenia
Lluis M. MirCNRS & Institut Gustave Roussy, Villejuif, France
Marie-Pierre RolsCNRS & IPBS, Toulouse, France
Gregor SeršaInstitute of Oncology, Ljubljana, Slovenia
Mounir TarekUniversity of Lorraine, Nancy, France
Justin TeissiéCNRS & IPBS, Toulouse, France
P. Thomas VernierOld Dominion University, Norfolk, Virginia, USA

Invited lecturers

Pouyan BoukanyDelft University of Technology, Delft, Netherlands
Maja ČemažarInstitute of Oncology, Ljubljana, Slovenia
Julie GehlHerlev Hospital, Herlev, Denmark
Antoni IvorraUniversity Pompeu Fabra, Barcelona, Spain
Henry JägerUniversity of Natural Resources and Life Sciences, Vienna, Austria
Tadej KotnikUniversity of Ljubljana, Slovenia
Rodney P. O'ConnorUniversity of Limoges, Limoges, France
Véronique PréatUniversity of Louvain, Brussels, Belgium

Founding faculty members

Damijan Miklavčič (University of Ljubljana) · Lluis M. Mir (CNRS & Institut Gustave Roussy) · Eberhard Neumann (University of Bielefeld) · Véronique Préat (Catholic University of Louvain) · Gregor Serša (Institute of Oncology, Ljubljana) · Justin Teissié (CNRS & IPBS, Toulouse)

Hands-on

Practical work & exercises

Wet-lab, computer-modelling and e-learning sessions. Participants selected up to three sessions from wet lab and modelling, plus computer simulation and e-learning. Exercise sheets (PDF) as distributed to participants.

S1	Laboratory safety	PDF ↓
L1	The influence of Mg²⁺ ions on gene electrotransfer efficiency	PDF ↓
L2	Monitoring cell membrane electroporation with ratiometric fluorescent dye Fura-2AM	PDF ↓
L3	Electropermeabilization detection with propidium iodide in plated cells	PDF ↓
L4	Measurements of the induced transmembrane voltage with fluorescent dye di-8-ANEPPS	PDF ↓
L5	Electrotransformation of Escherichia coli with plasmid DNA	PDF ↓
L6	Electroporation of planar lipid bilayers	PDF ↓
L7	E. coli inactivation in a continuous flow system	PDF ↓
L8	Analysis of electric field orientations on gene electrotransfer visualization at the membrane level	PDF ↓
L9	Skin electroporation	PDF ↓
C1	Treatment planning for electrochemotherapy and irreversible electroporation: optimization of voltage & electrode position	PDF ↓
C2	Numerical modelling of thermal effects during irreversible electroporation treatments	PDF ↓
C3	Molecular dynamics simulations of membrane electroporation	PDF ↓
E1	Electroporation of cells and tissues — interactive e-learning	PDF ↓

Abstracts & publication

Abstract instructions & proceedings

PDFInstructions for Abstract Submission DOCAbstract Template PDFJMB Special Issue — Guidelines for Authors

After presenting their work, participants were encouraged to submit a manuscript for consideration for publication in a special issue of the Journal of Membrane Biology, devoted to electroporation-based technologies and treatments, guest-edited by P. T. Vernier, L. M. Mir and D. Miklavčič. Participation to the workshop included attendance to lectures, book of abstracts and course material, lunches and social events, and certificate of attendance and examination (5 ECTS and 20 CME if required).

Electronic proceedings — all abstracts and course material.

Download proceedings (PDF)

Contacts

Organisation & contacts

Registration, abstract submission & other

Damijan Miklavčič
Faculty of Electrical Engineering, University of Ljubljana
Tržaška 25, 1000 Ljubljana, Slovenia
damijan.miklavcic@fe.uni-lj.si
tel +386 1 4768 456 · fax +386 1 4264 658

Accommodation & travel

Congress Agency Albatros
Mrs. Nina Bernard
nina@albatros-bled.com
tel +386 4 5780 350 · fax +386 4 5780 355